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2x ssofast evagreen supermix  (Bio-Rad)


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    Structured Review

    Bio-Rad 2x ssofast evagreen supermix
    2x Ssofast Evagreen Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 9239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2x+ssofast+evagreen+supermix/SsoFast+Eva+Green+Supermix/10__1093_slash_hr_slash_uhag140-315-17-25
    Average 99 stars, based on 9239 article reviews
    2x ssofast evagreen supermix - by Bioz Stars, 2026-10
    99/100 stars

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    Binding Assay:

    Article Title: MIXL1 activation in endoderm differentiation of human induced pluripotent stem cells
    Article Snippet: .. A sample mix was prepared as follows (per 96-well plate): 495 μL of 2X SsoFast EvaGreen SuperMix with low ROX (Biorad) and 49.5 μL 25X DNA Binding Dye (Fluidigm). ..

    Article Title: Establishing a biomarker set for the diagnosis of apple replant disease using qPCR
    Article Snippet: Following this, the pre-amplified cDNAs were purified 551 with exonuclease I (New England Biolabs GmbH, Germany), diluted 1:5 with DNA suspension buffer 552 pH 8.0 (Alpha Teknova, USA) and stored at -20°C until use. .. For the sample-mix, 2.7 μl of the pre-553 amplified and diluted cDNA was mixed with 3 μl 2x SsoFast EvaGreen supermix with low ROX 554 (Bio-Rad Laboratories, USA) and 0.3 μl 20x DNA binding dye sample loading reagent (Standard 555 BioTools, USA). .. The assay-mix consisted of 0.5 mM primer mix, 2.5 μl 2x assay loading reagent 556 (Standard BioTools, USA), and 2.25 μl DNA suspension buffer pH 8.0 (Alpha Teknova, USA).

    Article Title: Neuroprotective Potential of the Flavonoids Quercetin and Epicatechin in a C. elegans Tauopathy Model
    Article Snippet: Finally, exonuclease I was added to eliminate unincorporated primers at 37°C for 30 min and 85°C for 15 min. Preamplified cDNA was then used for RT‐qPCR reactions completed in a BioMark HD System (Fluidigm, South San Francisco, CA, USA). .. A 2.25 μL aliquot of each amplified cDNA was mixed with 2.5 μL of 2X SsoFast EvaGreen Supermix with Low ROX (Bio‐Rad) and with 0.25 μL of 20X DNA Binding Dye Sample Loading Reagent (Fluidigm). .. Individual RT‐qPCR primer pairs (100 μM) were diluted 1:10 with Tris‐EDTA (2.5 μL total volume) and mixed with 2.5 μL Assay Loading Reagent (Fluidigm).

    Article Title: Two pore domain THIK2 channel is involved in acute and chronic pain signal regulation
    Article Snippet: The assays mixes were prepared by mixing 2.5 μL of combined Primer (Forward/Reverse 10 μM) and 2.5 μl 2X Assay Loading Reagent (Fluidigm PN 100– 7611) to a final volume of 5 μL (per reaction). .. The samples pre-mixes were prepared by mixing 2.5 μL of 2X Ssofast EvaGreen Supermix (BioRad PN 172-5211), 0.25 μL 20X DNA Binding Dye (Fluidigm PN 100-7609) and 2.25 μL of diluted preamplified cDNAs to a final volume of 5 μL. .. The assays mixes were prepared by mixing 2.5 μL of combined Primer (Forward/Reverse 10 μM) and 2.5 μl 2X Assay Loading Reagent (Fluidigm PN 100– 7611) to a final volume of 5 μL (per reaction).

    Amplification:

    Article Title: Establishing a biomarker set for the diagnosis of apple replant disease using qPCR
    Article Snippet: Following this, the pre-amplified cDNAs were purified 551 with exonuclease I (New England Biolabs GmbH, Germany), diluted 1:5 with DNA suspension buffer 552 pH 8.0 (Alpha Teknova, USA) and stored at -20°C until use. .. For the sample-mix, 2.7 μl of the pre-553 amplified and diluted cDNA was mixed with 3 μl 2x SsoFast EvaGreen supermix with low ROX 554 (Bio-Rad Laboratories, USA) and 0.3 μl 20x DNA binding dye sample loading reagent (Standard 555 BioTools, USA). .. The assay-mix consisted of 0.5 mM primer mix, 2.5 μl 2x assay loading reagent 556 (Standard BioTools, USA), and 2.25 μl DNA suspension buffer pH 8.0 (Alpha Teknova, USA).

    Article Title: Neuroprotective Potential of the Flavonoids Quercetin and Epicatechin in a C. elegans Tauopathy Model
    Article Snippet: Finally, exonuclease I was added to eliminate unincorporated primers at 37°C for 30 min and 85°C for 15 min. Preamplified cDNA was then used for RT‐qPCR reactions completed in a BioMark HD System (Fluidigm, South San Francisco, CA, USA). .. A 2.25 μL aliquot of each amplified cDNA was mixed with 2.5 μL of 2X SsoFast EvaGreen Supermix with Low ROX (Bio‐Rad) and with 0.25 μL of 20X DNA Binding Dye Sample Loading Reagent (Fluidigm). .. Individual RT‐qPCR primer pairs (100 μM) were diluted 1:10 with Tris‐EDTA (2.5 μL total volume) and mixed with 2.5 μL Assay Loading Reagent (Fluidigm).

    Article Title: Identification of Functional Cellular Markers Related to Human Health, Frailty and Chronological Age.
    Article Snippet: The 10× assays mix was prepared by mixing 0.2 μL of 100 μM each Delta Gene primers (forward and reverse combined), 2 μL 2X Assay Loading Reagent (Fluidigm PN 100- 7611) and 1.8 μL of 1X DNA suspension buffer to a final volume of 4 μL (per reaction). .. The pre- sample mix was prepared by mixing 2 μL of 2X SsoFast EvaGreen Supermix with low ROX (Bio- Rad PN 172- 5211), 0.2 μL 192.24 Delta Gene Sample Reagent (Fluidigm PN 100–6653) and 1.8 μL pre- amplified and Exo I treated cDNA to a final volume of 4 μL. ..



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